This study examined the impacts of electron beam irradiation (0.25; 0,5; 1; 10 and 25 kGy) on bovine collagen hydrogels at a concentration of 3 wt%. The impact of irradiation was verified via the analysis of the mechanical, thermal and rheological properties, changes in the composition and structure of the collagen, its stability in the enzymatic environment, and its swelling and morphology. Both the degradation and cross-linking of the collagen mass were demonstrated in relation to the radiation dose. Degradation was proven via a decrease in the content of the triple helical part of the collagen and transformation into other collagen structural states, and cross-linking was demonstrated by an increase in the network chain density with the radiation dose. When compared with the non-irradiated gels, no significant qualitative changes in the configuration or ordering over longer distances were evident following the application of the irradiation doses. The apparent elastic modulus, overall thermal stability and rheological characteristics in the oscillatory regime, and the storage and loss moduli of elasticity correlated positively with the radiation dose.

Publication:
M. Šupová, T. Suchý, M. Vopálková, P. Novotná, M. Šulc, Z. Hrdlička, M. Houška (2025). The electron beam irradiation of type I bovine collagen gels: Insights into radiation dose and water content impacts on the composition and the structural, rheological, thermal and mechanical properties. International Journal of Biological Macromolecules 320, 146099. https://doi.org/10.1016/j.ijbiomac.2025.146099

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Publication:
M. Bujda, T. Suchý, V. Herynek, J. Dušková, M. Žaloudková, L. Šefc, K. Klíma, R. Plachý, R. Foltán (2025). In Vivo Degradation and Local Tissue Response of Experimental Carp Collagen Membranes: Micro-MRI and Histological Analysis. Biopolymers 116, e70045, https://doi.org/10.1002/bip.70045

Publication:
J. Štípek, H. Rohm, T. Suchý, M. Šupová, R. Žitný, J. Skočilas, M. Žaloudková, M. Houška (2025). Characterization of structural properties and non-linear rheological behavior of collagen over a wide range of mass fractions under LAOS. Polymer Engineering and Science 65(6):3018–3028. https://doi.org/10.1002/pen.27195

Publication:
A. Malhocká, M. Švábová, T. Suchý (2025). Chemotaxonomy according to leaf terpenes of eight cypress species growing in the Czech Republic in the light of modern phylogenetic classification. Biochemical Systematics and Ecology 120, 104980. https://doi.org/10.1016/j.bse.2025.104980

Publication:
L. Klecandová, A. Slíva, M. Šindler, M. Šupová, S. Kumar Sathish, J. Kupková, L. Hružík, K. Lafdi, K. Čech Barabaszová, G. Simha Martynková (2025). Effect of Processing on Structural and Morphological Changes of Hydroxyapatite-Based Materials. ChemistrySelect 10, e202405109, https://doi.org/10.1002/slct.202405109

Publication:
Horný, L. Vištejnová, T. Suchý, E. Košťáková Kuželová, H. Chlup, J. Kronek, Z. Sobotka, Z. Petřivý, M. Žaloudková, K. Havlíčková, J. Moláček (2025). Degradation of the mechanical properties of poly-lactide-caprolactone-collagen composite for pulmonary artery banding after implantation into a rat's peritoneum. Medical Engineering & Physics 138, 104319. https://doi.org/10.1016/j.medengphy.2025.104319

Publication:
M. Šupová, Š. Rýglová, T. Suchý, M. Žaloudková, M. Braun (2025). The Impact of GAGs, Cross-Link Maturity and Telopeptides on the Formation of a Porcine Collagen-Based Hydrogel. Gels 11, 695. https://doi.org/10.3390/gels11090695

Publication:
D. Řimnáčová, I. Perná, M. Novotná, M. Šupová, M. Nováková, O. Bičáková (2025). Activated Carbon–Geopolymer Composites: Influence of Particle Size and Content on CO2 Adsorption and Mechanical and Thermal Properties. Crystals 15, 892. https://doi.org/10.3390/cryst15100892

Various studies have correlated the mechanical properties of the aortic wall with its biochemical parameters and inner structure. Very few studies have addressed correlations with the cohesive properties, which are crucial for understanding fracture phenomena such as aortic dissection, i.e. a life-threatening process. Aimed at filling this gap, we conducted a comprehensive biochemical and histological analysis of human aortas (the ascending and descending thoracic and infrarenal abdominal aorta) from 34 cadavers obtained post-mortem during regular autopsies. 

Publication:
Suchý, T.; Horný, L.; Šupová, M.; Adámek, T.; Blanková, A.; Žaloudková, M.; Grajciarová, M.; Yakushko, Y.; Blassová, T.; Braun, M. Age-related changes in the biochemical composition of the human aorta and their correlation with the delamination strength,
Acta Biomaterialia 2024, In Press, Corrected Proof. doi.org/10.1016/j.actbio.2024.11.002

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Magnesium is an excellent material in terms of biocompatibility and its corrosion products can serve as an active source for new bone formation. However, localized corrosion and H2 generation limit the potential of Mg-based implants. Utilizing low-alloyed Mg-Zn wires can strongly reduce problems with large H2 bubbles and improve the mechanical properties considerably while maintaining excellent long-term biocompatibility. Acidic pickling and a polymer coating can be effectively used to lower the rate of in vivo degradation. In this work, microstructural, mechanical, and in vitro characterization of 250 µm and 300 µm extruded wires made from ultra-pure Mg, commercially pure Mg, Mg-0.15Zn, Mg-0.4Zn and Mg-1Zn was performed. Additionally, Mg-0.4Zn wires together with a variant coated with a copolymer of l-lactide and ε-caprolactone were tested in vivo on artificially damaged Wistar rat femurs. Based on the observed Mg-induced osteogenesis, polymer-coated Mg wires with a small addition of Zn are a perspective material for bone-support applications, such as cerclage and fixation wires.

Publication:
Tesař, K.; Luňáčková, J.; Jex, M.; Žaloudková, M.; Vrbová, R.; Bartoš, M.; Klein, P.;  Vištejnová, L.; Dušková, J.; Filová, E.; Sucharda, Z.; Steinerová, M.; Habr, S.; Balík, K.;  Singh, A. (2024): In vivo and in vitro study of resorbable magnesium wires for medical implants:  Mg purity, surface quality, Zn alloying and polymer coating, Journal of Magnesium and  Alloys 2024, 12, 2472-2488. doi.org/10.1016/j.jma.2024.06.003

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New biomaterials are routinely evaluated for their degradation behaviour in the real body environment. Following the 3R strategy, in vitro simulated body conditions are often preferred. No studies that simultaneously compare such conditions with the real body environment have been conducted to date. Model porous collagen scaffolds were exposed for 21 days to eight different environments: simple salt-based and enzymatic media, human blood plasma, cell culture media with and without human fibroblasts and ex vivo model cortical bone, and subsequently compared with an in vivo environment represented by a pig peritoneum. The mechanical properties of the scaffolds were then determined via uniaxial compression testing, and the structural properties via the micro-CT, weight loss, infrared spectroscopy, X-ray diffraction and histological methods. Interestingly, the various analysed simulated body conditions caused differing alterations in the collagen scaffold characteristics when compared with the real body environment. The mechanical properties were similar during the first 7 days of incubation but diverged after 14 and 21 days. The structural properties varied significantly after just 7 days of incubation. The histological evaluation of the scaffolds exposed to the cellular, ex vivo and in vivo conditions revealed the poor ability of cells to completely populate the scaffolds, accompanied by the massive ingrowth of connective tissue into the in vivo exposed scaffolds, which resulted in their variable global behaviour. In conclusion, the value of in vitro simulated body environments lies in their screening capacity and feasibility; however, direct extrapolation to real body conditions needs to be verified going forward.

Publication:
Suchý, T.; Vištejnová, L.; Sedláček, R.; Vopálková, M.; Šupová, M.; Simha Martynková, G.; Staňo, J.; Klein, P.; Denk, F.; Kužma, J.; Bartoš, M. The comparison of eight different common in vitro and ex vivo environments with in vivo conditions applying model collagen samples: Correlation possibilities and their limits, Polymer Testing 2024, 140, 108621. https://doi.org/10.1016/j.polymertesting.2024.108621

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Publication:
Trávníčková, M.; Filová, E.; Slepička, P.; Slepičková Kasalková, N.; Kocourek, T.; Žaloudková, M.; Suchý, T.; Bačáková, M. (2024) Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells, International Journal of Molecular Sciences, 25(5), 2837. https://doi.org/10.3390/ijms25052837

Publication:
Kužma, J.; Suchý, T.; Horný, L.; Šupová, M.; Sucharda, Z. Comparative Study of the Dehydrothermal Crosslinking of Electrospun Collagen Nanofibers: The Effects of Vacuum Conditions and Subsequent Chemical Crosslinking, Polymers 2024, 16(17), 2453.
https://doi.org/10.3390/polym16172453

Publication:
Perná I., Zárybnická L., Mácová P., Šupová M., Ševčík R. (2024): Physico-mechanical properties of geopolymers after thermal exposure: Influence of filler, temperature and dwell time. Construction and Building Materials 451, 138893. https://doi.org/10.1016/j.conbuildmat.2024.138893

Publication:
Kanoková D., Matějka R., Žaloudková M., Zigmond J., Šupová M., Matějková J. (2024). Active Media Perfusion in Bioprinted Highly Concentrated Collagen Bioink Enhances the Viability of Cell Culture and Substrate Remodeling. Gels 10, 316. https://doi.org/10.3390/gels10050316

Publication:
Matějková J., Kanoková D., Šupová M., Matějka R. (2024). A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation. Gels 10, 66. https://doi.org/10.3390/gels10010066

The present invention relates to a method of preparation of a degradable sandwich collagen sponge with hemostatic effects, controlled degradation time and capable of controlled local release of active substances, for example antibiotics for use in wound dressings in surgery, orthopedics, traumatology and plastic surgery, comprising a low porous collagen core and highly porous collagen sponge as peripheral parts.

Publication:
T. Grus, T. Suchý, M. Šupová, H. Chlup, J. Hartinger (2023): Sandwich and composite collagen sponge for controlled release of active substances, and method of preparation thereof. EP 3 838 302, European Patent Office, date of publication 23.08.2023.
 

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Procedures for processing and analysing collagen samples were evaluated and a study was carried out to provide a comprehensive description of the suitability of different methods of collagen isolation from a wide range of animal species and tissues in terms of reproducibility, quality and composition.

Publication: 
Š. Rýglová, M. Braun, T. Suchý, M. Hříbal, M. Žaloudková, L. Vištějnová (2023): The investigation of batch-to-batch variabilities in the composition of isolates from fish and mammalian species using different protocols. Food Research International 169, 112798. doi.org/10.1016/j.foodres.2023.112798
 

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The aim was to describe the impact of collagen in the gel and dry state to various doses of electron beam radiation (1, 10 and 25 kGy) which are using for food processing and sterilization Irradiation of collagen in different states has different effects: irradiation in the dry state mainly causes the breakdown of collagen molecules; irradiation of the gel with increasing doses causes a change in its mechanical properties, in particular an increase in its elastic modulus. Irradiation increases the cross-linking of scleroprotein molecules (with only partial degradation), but does not alter the composition and morphology of collagen, either in the gel or in the dry state.
The result was achieved in collaboration with the Faculty of Mechanical Engineering of the Czech Technical University in Prague, the Food Research Institute Prague, Nuclear Physics Institute CAS Řež and University of Chemistry and Technology Prague.

Publication:
M. Šupová, T. Suchý, H. Chlup, M. Šulc, T. Kotrč, L. Šilingová, M. Žaloudková, Š. Rýglová, M. Braun, D. Chvátil, Z. Hrdlička, M. Houška (2023): The electron beam irradiation of collagen in the dry and gel states: The effect of the dose and water content from the primary to the quaternary levels. International Journal of Biological Macromolecules 253 (2023) 126898. doi.org/10.1016/j.ijbiomac.2023.126898

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Fig. 6. Representative SEM images of the collagen gel before irradiation (0 kGy) and the collagen gels irradiated with doses of 1, 10 and 25 kGy. The images in the left column were taken at a magnification of 20,000× (the bar represents 5 μm), and those in the right column at a magnification of 50,000× (the bar represents 2 μm).

Publication:
E. Filova, M. Šupová, A. Eckhardt, M. Vrbacky, A. Blanquer, M. Trávničková, J. Knitlová, T. Suchý, S. Rýglová, M. Braun, Z. Burdíková, M. Schätz, V. Jenčová, M. Lisnenko, L. Behalek, R. Procházková, R. Sedláček, K. Kubasová, L. Bačáková (2023): Adipose-Derived Stem Cells in Reinforced Collagen Gel: A Comparison between Two Approaches to Differentiation towards Smooth Muscle Cells, International Journal of Molecular Sciences. 24(6) 5692. doi.org/10.3390/ijms24065692

Publication:
M. Houška, A. Landfeld, P. Novotná, J. Strohalm, M. Šupová, T. Suchý, H. Chlup, J. Skočilas, J. Štípek, M. Žaloudková, M. Šulc (2023): Properties of Bovine Collagen as Influenced by High-Pressure Processing. Polymers 15(11):2472. doi.org/10.3390/polym15112472

Publication:
H. Chlup, T. Suchý, M. Šupová (2023): The electron beam induced cross-linking of bovine collagen gels with various concentrations: The mechanical properties and secondary structure, Polymer 287, 126423. doi.org/10.1016/j.polymer.2023.126423

Publication:
M. Šupová, T. Suchý, H. Chlup, J. Štípek, R. Žitný, A. Landfeld, J. Skočilas, M. Žaloudková, Š. Rýglová, M. Braun, J. Štancl, M. Houška (2023): The comprehensive evaluation of two collagen gels used for sausage casing extrusion purposes: The role of the structural and mechanical properties, Journal of Food Engineering 343 , 111387. doi.org/10.1016/j.jfoodeng.2022.111387

Publication:
C.M. Saratti, N. Scotti, A. Comba, J. Bijelic-Donova, T. Suchý, M. Abdelaziz, J.G. Leprince, G.T. Rocca (2023): Exploring the influence of placing bi-directional E-glass fibers as protective layer under a CAD-CAM resin composite on the fracture pattern. Dental Materials 39, 986–993. doi.org/10.1016/j.dental.2023.09.003

Publikace:
L. Bačáková, E. Filová, Š. Pražák, I. Vacková, T. Suchý, M. Šupová, J. Šepitka, R. Procházková, V. Jenčová, E. Kuželová Košťáková, M. Lisnenko, D. Lukáš, J. Valtera (2023): Vyztužený kompozitní hydrogel s buňkami. (Reinforced composite hydrogel with cells.) Užitný vzor č.: 37438.

Machoň, V.; Bartoš, M.; Suchý, T.; Levorová, J.; Foltán, R.: Micro-computed tomography evaluation of bone architecture in various forms of unilateral condylar hyperplasia. Journal of Oral and Maxillofacial Surgery. 2022, DOI: doi.org/10.1016/j.ijom.2022.05.008

Stepanovska, J.; Otahal, M.; Hanzalek, K.;Supova, M.; Matejka, R.: pH Modification of High-Concentrated Collagen Bioinks as a Factor Affecting Cell Viability, Mechanical Properties, and Printability. Gels 2021, 7, 252. doi.org/10.3390/gels7040252

Šupová, M.: Problems associated with the assessment of organic impuritiesin bioapatites isolated from animal sources: a review. Journal of the Australian Ceramic Society, 2022, 58 (1), 227 - 247. doi.org/10.1007/s41779-021-00678-y

Straka, P.; Bičáková, O.; Šupová, M. : Slow pyrolysis of waste polyethylene terephthalate yielding paraldehyde, ethylene glycol, benzoic acid and clean fuel. Polymer Degradation and Stability 198 (2022) 109900. https://doi.org/10.1016/j.polymdegradstab.2022.109900

Pazourková, L.; Martynková, G.S.; Šupová, M.: Ca-deficient hydroxyapatite synthesis on the bioapatite bovine bone substrate study. Materials Today: Proceedings 52 (2022) 227–231. DOI: doi.org/10.1016/j.matpr.2021.11.412 

Publication:
Wolfová, Lucie - Šenková, A. - Suchý, Tomáš - Šupová, Monika - Karkoška, J. - Forostyak, S.: Medical device based on bioapatite and demineralized bone matrix for bone defect augmentation, 2021: Vlastník: Ústav experimentální medicíny AV ČR. Datum udělení vzoru: 2.2.2021. Číslo vzoru: 34826, Institucionální podpora: RVO:68378041; RVO:67985891

Mizera, J., Řanda, Z., Suchý, V., Strunga, V., Klokočník, J., Kostelecký, J., Bezděk, A., Moravec, Z., 2021. Parent crater for Australasian tektites beneath the sands of the Alashan Desert, Northwest China: Best candidate ever?, in Foulger, G.R., Hamilton, L.C., Jurdy, D.M., Stein, C.A., Howard, K.A., and Stein, S., eds., In the Footsteps of Warren B. Hamilton: New Ideas in Earth Science: Geological Society of America Special Paper 553, p. 323–334 DOI: doi.org/10.1130/2021.2553(25)