The most important expected result of the project is a selection sites located in protected landscape territories of Czech Republic where undisturbed natural soils on the different parent materials of the different positions can be found and protected as a long term standard suitable for monitoring of environmental changes (e.g. soil acidification and erosion, acid rains, eutrophication, aerosol deposition). The previous Holocene environmental and climatic impact will be characterised by ensenble of doil analysis, first of all by micromorphology, further by mineralogy including clay mineralogy, by malacozoology and geomorphology. The evidence of anthropogenic factor in the evolution of soil in Holocene will be obtained by comparison between neighbouring natural and agricultural soil cover evolution will be help to compare recent and fossil soils whose evolution passed with no human influence and is covered by various sediments.