Department of Composites and Carbon Materials
Institute of Rock Structure and Mechanics of the CAS

Services & Expertise International Cooperation
Basic Information Research Activities Most important results Projects

Vancomycin-Loaded Collagen/Hydroxyapatite Layers Electrospun on 3D Printed Titanium Implants Prevent Bone Destruction Associated with S. epidermidis Infection and Enhance Osseointegrationi


Graphical abstract


The aim of the study was to develop an orthopedic implant coating in the form of vancomycin-loaded collagen/hydroxyapatite layers (COLHA+V) that combine the ability to prevent bone infection with the ability to promote enhanced osseointegration. The ability to prevent bone infection was investigated employing a rat model that simulated the clinically relevant implant-related introduction of bacterial contamination to the bone during a surgical procedure using a clinical isolate of Staphylococcus epidermidis.

Publication: T. Suchý, L. Vištejnová, M. Šupová, P. Klein, M. Bartoš, Y. Kolinko, T. Blassová, Z. Tonar, M. Pokorný, Z. Sucharda, M. Žaloudková, F. Denk, R. Ballay, Š. Juhás, J. Juhásová, E. Klapková, L. Horný, R. Sedláček, T. Grus, Z. Čejka, Z. Čejka, K. Chudějová, J. Hrabák, Vancomycin-Loaded Collagen/Hydroxyapatite Layers Electrospun on 3D Printed Titanium Implants Prevent Bone Destruction Associated with S. epidermidis Infection and Enhance Osseointegration, Biomedicines. 9 (2021) 531. DOI: 10.3390/biomedicines9050531, IF 6,081, Q1 32/275, JCR 2020 www.webofscience.com


Representative micro-CT and histological images of identical samples in the COLHA+V implants without the application of S. epidermidis (upper line), COLHA implants with S. epidermidis (middle line), and COLHA+V with S. epidermidis (bottom line) groups



Representative images of the titanium implants. Ti-printed implant before (A) and after (B) the deposition of a COLHA+V layer intended for use in the antimicrobial activity experiment; Ti -printed implant before (C) and after (D) the deposition of a COLHA+V layer intended for use in the osseointegration experiment; Ti-printed control sample (E).


Further important publications and outputs, patents

T. Suchý, M. Bartoš, R. Sedláček, M. Šupová, M. Žaloudková, G.S. Martynková, R. Foltán, Various simulated body fluids lead to significant differences in collagen tissue engineering scaffolds, Materials (Basel). 14 (2021) 1–17. DOI: 10.3390/ma14164388. IF 3,623, Q1 (17/80), JCR 2020 www.webofscience.com

M. Braun, Š. Rýglová, T. Suchý, Determination of glycosaminoglycans in biological matrices using a simple and sensitive reversed-phase HPLC method with fluorescent detection, J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 1173 (2021). DOI: 10.1016/j.jchromb.2021.122626. IF 3,205, Q2 (36/83), JCR 2020 www.webofscience.com

S. Ryglova, M. Braun, M. Hribal, T. Suchy, D. Voros, The proportion of the key components analysed in collagen-based isolates from fish and mammalian tissues processed by different protocols, J. FOOD Compos. Anal. 103 (2021). DOI: 10.1016/j.jfca.2021.104059. IF 4,556, Q1 (30/144), JCR 2020 www.webofscience.com

M. Krticka, L. Planka, L. Vojtova, V. Nekuda, P. Stastny, R. Sedlacek, A. Brinek, M. Kavkova, E. Gopfert, V. Hedvicakova, M. Rampichova, L. Kren, K. Liskova, D. Ira, J. Dorazilova, T. Suchy, T. Zikmund, J. Kaiser, D. Stary, M. Faldyna, M. Trunec, Lumbar Interbody Fusion Conducted on a Porcine Model with a Bioresorbable Ceramic/Biopolymer Hybrid Implant Enriched with Hyperstable Fibroblast Growth Factor 2, Biomedicines. 9 (2021). DOI: 10.3390/biomedicines9070733. IF 6,081, Q1 32/275, JCR 2020www.webofscience.com

M. Krticka, V. Nekuda, D. Ira, R. Sedlacek, T. Suchy, M. Kavkova, A. Brinek, E. Gopfrt, A. Bilik, J. Kaiser, L. Kren, L. Planka, Microcomputed tomographic, biomechanical and histological analyses of lumbar interbody fusion with iliac crest bone graft in a pig model, ACTA Vet. BRNO. 90 (2021) 69–75. DOI: 10.2754/avb202190010069. IF 0,667, Q4 112/146, JCR 2020 www.webofscience.com

P. Chocholata, V. Kulda, J. Dvorakova, M. Supova, M. Zaloudkova, V. Babuska, In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels, International Journal of Molecular Sciences, 22(17) (2021) 9335. DOI: 10.3390/ijms22179335. IF 5.924, Q1 67/295, JCR 2020 www.webofscience.com www.asep.lib.cas.cz

J. Stepanovska, M. Supova, K. Hanzalek, A. Broz, R. Matejka, Collagen Bioinks for Bioprinting: A Systematic Review of Hydrogel Properties, Bioprinting Parameters, Protocols, and Bioprinted Structure Characteristics, Biomedicines, 9(9) (2021) 1137. DOI: 10.3390/biomedicines9091137. IF 6.081, Q1 32/275, JCR 2020 www.webofscience.com www.asep.lib.cas.cz

I. Perná, M. Novotná, M. Šupová, T. Hanzlíček, Beach Sand: Alternative Filler in Metakaolin-Based Geopolymers, Journal of Materials in Civil Engineering, 33(4) (2021) 04021017. DOI:10.1061/(ASCE)MT.1943-5533.0003641. IF 3.266, Q2 (45/137, 67/172, 29/87) JCR 2020 www.webofscience.com www.asep.lib.cas.cz

I. Perná, M. Novotná, D. Řimnáčová, M. Šupová, New Metakaolin-Based Geopolymers with the Addition of Different Types of Waste Stone Powder, Crystals, 11(8) (2021) 983. DOI:10.3390/cryst11080983. IF 2.589, Q2 (9/25, 148/384, 13/33) JCR 2020 www.webofscience.com www.asep.lib.cas.cz

M. Černý, Z. Chlup, A. Strachota, J. Brus, J. Schweigstillová, Š. Rýglová, O. Bičáková.: In-situ measurement of mechanical properties and dimensional changes of preceramic thermosets during their pyrolysis conversion to ceramics using thermomechanical analysis. Ceramics International, 47(16) (2021). IF 4,53 Q1 (3/29) (2020) DOI:10.1016/J.CERAMINT.2021.05.041

Z. Chlup, M. Černý, A. Strachota, Š. Rýglová, J. Schweigstillová, J. Svítilová, L. Trško, B. Hadzima, Effect of pyrolysis temperature on the behaviour of environmentally friendly hybrid basalt fibre reinforced composites, Applied Composite Materials, přijato do tisku IF 2,181 Q3 (19/28) (2020) DOI:10.1007/s10443-021-09990-z

Russian patent Suchy, T.; Supova, M.; Denk, F.; Rýglová, Š.; Žaloudková, M.; Sucharda, Z.; Ballay, R.; Horný, L.; Čejka, Z.; Pokorný, M.; Knotková, K.; Velebný, V.: Нанокомпозитный слой на основе коллагеновых нановолокон и способ его изготовления. ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ. RU 2 756 164. 2021-09-28. www.patents.s3.yandex.net

National patent Grus, T.; Suchy, T.; Supova, M.; Chlup, H.; Hartinger, J.: Sandwich collagen foam for controlled release of active substances and preparing it. Industrial Property Office of the Czech Republic. Patent 308862. 2021-07-21.www.isdv.upv.cz

List of publications and results in 2021
List of publications and results 2015 - 2020

Further publications in ASEP database